Products Description

This kit uses polymerase chain reactor-fluorescent probe technology to design specific primers according to the nucleic acid sequence of monkeypox virus for amplification of corresponding nucleic acid fragments. At the same time, the highly specific probe can be combined with the corresponding nucleic acid fragment and hydrolyzed under the action of exonuclease activity to generate fluorescence signal for detection. Real-time amplification curve can be obtained according to the relationship between fluorescence signal and amplification cycle number.
How is monkeypox transmitted?
The virus transmitted from animal to human is by direct contact with the blood, bodily fluids, or cutaneous or mucosal lesions of infected animals.
From human to human is by close contact with respiratory secretions, skin lesions of an infected person or recently contaminated objects.
Most reported cases so far have been identified through sexual health or other health services in primary or secondary health-care facilities and have involved mainly men who have sex with men.
specification
|
Technology |
Polymerase chain reactor-fluorescent probe technology |
|
Specification |
25 tests/box, 50tests/box |
|
Specimen Types |
Serum and human pustular or vesicular rash specimens |
|
lnternal Control |
Exogenous internal control, not limited to human clinical samples |
|
PCR turnaround time |
36 mins |
|
Limit of Detection |
400 copies/mL |
|
Precision |
CV≤5% |
|
Storage Temperature |
-20±5℃ |
|
Shelf Life |
12 Months |
|
Applied PCR Machine |
1.Applied BiosystemsTM 7500 real-time PCR system; 2.ABI 7500 Real-time fluorescence quantitative PCR instrument; 3.SLAN®-96S/96P real-time PCR system; 4.Thermofisher QuantStudioTM5 Real-time fluorescence quantitative PCR; 5.MA-6000 Real-Time Quantitative Thermal Cycler |
Kit component
|
No. |
Components |
Volume/25T |
Volume/50T |
Main Components |
|
1 |
MPV-PCR detection Mix |
950μL/tube, 1tube |
950μL/tube, 2tubes |
Primers, probes, dNTPs, PCR buffer etc. |
|
2 |
MPV-Enzyme mixture |
50μL/tube,1tube |
100μL/tube,1tube |
H-Taq, UNG |
|
3 |
MPV-Positive control product |
500μL/tube,1tube |
500μL/tube,1tube |
Cloning Plasmids for Target Gene Fragments |
|
4 |
MPV-Negative control product |
500μL/tube,1tube |
500μL/tube,1tube |
Sterilized saline |
performance index
1. Specificity: The kit has no cross-reaction with measles virus, rubella virus, parvovirus B19, varicella zoster virus, syphilis virus and herpes simplex virus.
2. Minimum detection limit: 400 copies/mL.
3. Precision: The coefficient of variation (CV %) of the Ct value of the intra-assay precision is less than or equal to 5%.
4. Interfering substances that may exist in the sample: hemoglobin (≤2mg/dL), total bilirubin (≤28mg/dL), triglyceride (≤3g/dL), total IgG (≤40mg/mL) have no effect on the detection. Influence; paracetamol (≤60μg/mL) has no influence on the detection.
certification

precaution
1.This kit is for professional use only.
2.Standard precautions should be followed. All patient samples and positive controls should be considered potentially infectious and handled accordingly.
3.Handle hazardous or biocontaminated materials in accordance with your institution's practices.
4.Please read the instructions carefully before operation. This kit is only for emergency use in in vitro diagnostic testing. During the experiment, from specimen collection and storage to laboratory testing, each step of the operation should be strictly in accordance with relevant biosafety regulations and molecular experiments.
5.A separate laboratory area is required for the execution of predetermined procedures. a) Area 1: preparation area-preparing detection reagents b)Area 2: sample processing area-processing samples and controls c) Area 3:amplification area-PCR reaction area.
6.All test samples must be treated as infectious substances. Change lab coats and disposable gloves to avoid cross-contamination between samples. Disposal of samples and waste must comply with local, state and national regulations.
FAQ
Q1: Can I have a sample for our testing?
A1: Yes, stock samples are available.
Q2: What is sample ready time?
A2: Usually 3-5 days, since it was produced against specific demands to guarantee most freshness.
Q3: What about the payment methods?
A3: L/C, T/T, Paypal, Western Union
Q4: What's your delivery time?
A4: Usually 3-35 days after receiving the deposit. OEM package takes around 30 days.
Q5: Can I print our logo on the package?
A5: Yes, we can do custom printing on pouches and boxes.
Q6: Are you a factory or trading company?
A6: We are Manufacturer.
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